%0 Journal Article
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%@nexthigherunit 8JMKD3MGPCW/3ESR3H2
%@archivingpolicy denypublisher denyfinaldraft12
%X The combination of low friction, wear resistance, high hardness, biocompatibility, and chemical inertness makes diamond-like carbon (DLC) films suitable in numerous applications in biomedical engineering. The cytotoxicity of DLC films containing TiO2 nanoparticles was practical and theoretically evaluated. The films were grown on 316L stainless steel substrates from a dispersion of TiO2 nanopowder in hexane. Raman spectroscopy shows that the presence of TiO2 increased the graphite-like bonds in the films. The incorporation of TiO2 nanoparticles into DLC films increases surface roughness, decreases water contact angle (increased hydrophilic character), and increases the total free surface energy due to the higher polar component. As the concentration of TiO2 increased, the films increased the cell viability (MTT assay), becoming more thermodynamically favorable to cell spreading (DFAdh values became more negative). This was evidenced through the increasing number of projections (philopodia and lamellipodia), indicating a higher adhesion between the L929 cells and the films. The practical and theoretical findings of this study show that the incorporation of TiO2 into DLC films is effective in enhancing cell viability. These results show the potential use of DLC and TiO2-DLC films in biomedical applications.
%8 Dec.
%N 4-6
%T Thermodynamic aspects of fibroblastic spreading on diamond-like carbon films containing titanium dioxide nanoparticles
%@electronicmailaddress
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%@electronicmailaddress
%@electronicmailaddress vladimir@las.inpe.br
%@secondarytype PRE PI
%K Cell spreading, Cellular viability, Diamond-like carbon, Hydrophilicity, TiO2 nanoparticles, Work of adhesion.
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%@usergroup lattes
%@usergroup secretaria.cpa@dir.inpe.br
%@group
%@group
%@group
%@group LAS-CTE-INPE-MCT-BR
%@e-mailaddress vladimir@las.inpe.br
%3 marciano1.pdf
%@secondarymark B1_ASTRONOMIA_/_FÍSICA B1_ENGENHARIAS_IV B3_MATEMÁTICA_/_PROBABILIDADE_E_ESTATÍSTICA B1_QUÍMICA
%F lattes: 3455204481678421 5 MarcianoMaWaLoTrSoSi:2011:ThAsOf
%@issn 1432-881X
%2 dpi.inpe.br/plutao/2011/09.22.19.28.03
%@affiliation Laboratorio de Nanotecnologia Biomedica (NanoBio), Universidade do Vale do Paraiba (Univap), Av. Shishima Hifumi 2911, Sao Jose dos Campos, SP 12244-000, Brazil
%@affiliation Laborato´rio de Biologia Celular e Tecidual, Universidade do Vale do Paraiba (Univap), Av. Shishima Hifumi 2911, Sao Jose dos Campos, SP 12244-000, Brazil
%@affiliation Laboratorio de Nanotecnologia Biomedica (NanoBio), Universidade do Vale do Paraiba (Univap), Av. Shishima Hifumi 2911, Sao Jose dos Campos, SP 12244-000, Brazil
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Laborato´rio de Biologia Celular e Tecidual, Universidade do Vale do Paraiba (Univap), Av. Shishima Hifumi 2911, Sao Jose dos Campos, SP 12244-000, Brazil
%B Theoretical Chemistry Accounts
%@versiontype publisher
%P 1085-1093
%4 dpi.inpe.br/plutao/2011/09.22.19.28
%@documentstage not transferred
%D 2011
%V 130
%@doi 10.1007/s00214-011-1018-5
%A Marciano, Fernanda Roberta,
%A Wachesk, Cristiane da Costa,
%A Lobo, Anderson de Oliveira,
%A Trava-Airoldi, Vladimir Jesus,
%A Soares, Cristina Pacheco,
%A Silva, Newton Soares da,
%@dissemination WEBSCI; PORTALCAPES.
%@area FISMAT