%0 Journal Article
%4 sid.inpe.br/plutao/2020/06.16.01.41
%2 sid.inpe.br/plutao/2020/06.16.01.41.27
%@doi 10.1007/s42452-020-1967-1
%@issn 2523-3963
%F lattes: 3455204481678421 11 HurtadoWQMFTDSMHAT:2020:SiPrOb
%T A simple procedure to obtain nanodiamonds from leftover of HFCVD system for biological application
%D 2020
%9 journal article
%A Hurtado, Carolina Ramos,
%A Wachesk, Cristiane da Costa,
%A Queiroz, Rafaela Campos,
%A Macedo, Erenilda Ferreira de,
%A Correia, Rebeca Falcão Borja de Oliveira,
%A Taiariol, Thalita Sani,
%A Diniz, Milton Faria,
%A Santos, Alexandre Martins Isaias dos,
%A Montanheiro, Thais Larissa do Amaral,
%A Hurtado, Gabriela Ramos,
%A Trava-Airoldi, Vladimir Jesus,
%A Tada, Dayane Batista,
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Instituto de Aeronáutica e Espaço (IAE)
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@affiliation Instituto Tecnológico de Aeronáutica (ITA)
%@affiliation Universidade Estadual Paulista (UNESP)
%@affiliation Instituto Nacional de Pesquisas Espaciais (INPE)
%@affiliation Universidade Federal de São Paulo (UNIFESP)
%@electronicmailaddress carolina.hurtado@ifsp.edu.br
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress rebeca.correia@inpe.br
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress
%@electronicmailaddress vladimir.airoldi@inpe.br
%B SN Applied Sciences
%V 2
%N 352
%P 2
%K Nanodiamonds · Ultrasonic cavitation · CVD diamond · Functionalization · Cytotoxicity · Colloidal suspension.
%X Nanodiamonds (NDs) are amongst the most investigated carbon-based nanostructures due to their chemical stability and favorable mechanical properties. Despite the number of works on methods for NDs production, one of the main challenges is to achieve their colloidal stability in aqueous suspension. Additionally, NDs are normally obtained by expensive, complex and time-consuming process. Herein, it was presented a facile method to obtain NDs in aqueous suspension by using columnar structure diamond from Hot-Filament Chemical Vapour Deposition reactor (HFCVD). CVD diamond leftover thick flm from CVDVale Company was used. Therefore, this method has the advantage of being not only practical but also cost-efective since it brings a proftable use of CVD diamond leftover. The Diamond thick flm was submitted to ultrasonic cavitation in the presence and absence of ZrO2 microbeads in aqueous medium. The NDs hydrodynamic diameter and the stability in aqueous suspension were monitored by light scattering, size and morphology were analyzed by transmission electronic microscopy. Considering the wide application of NDs in biomedical devices, cytotoxicity of aqueous suspensions of NDs was evaluated against murine embryonic fbroblast cells. Furthermore, NDs were functionalized with hydrogen and carboxyl groups. NDs aqueous suspension of straight size distribution was obtained even in the absence of ZrO2 beads, indicating that they may be dispensable in order to decrease NDs size. NDs of average hydrodynamic diameter of 22 nm and −35 mV of Zeta-potential were obtained after ultrasonic cavitation followed by 2 h of centrifugation, not demonstrating cytotoxicity to cells at very low (0.050.5 μg/mL) nor at higher concentrations (116 μg/mL). Nevertheless, NDs showed a moderate cytotoxicity at intermediary concentration range (0.52.2 μg/mL). From our knowledge, this is the frst work that reports on a facile method for providing NDs aqueous suspension with high colloidal stability from HFCVD diamond leftover.
%@language pt
%3 hurtado_simple.pdf